ECIS Application Webinar Series

 
To register for a webinar please go to: https://appliedbiophysics.webex.com

ECIS is used in over 160 labs worldwide and ECIS data has been published in a growing list of over 400 peer-reviewed publications.

ECIS electrically monitors cell morphological changes and provides quantitative, real-time measurements of cell motility, attachment and spreading, and barrier function. Experimental protocols include studies of cell-integrin interactions, metastatic invasion, signal transduction, and cell migration (via automated wound/healing).

The ECIS Application Webinar series will review the topics listed below in 20 minute, web-based, interactive seminars. To view a pre-recorded video of the Introduction to ECIS click here: ECIS Introduction (11:02)

Please contact Applied BioPhysics at info@biophysics.com  to obtain a copy of the power point slides used in a webinar presentation

All Webinars are presented by Dr. Charles Keese, President Applied BioPhysics.

2014 WEBINAR SCHEDULE

ECIS Theory – 11:00 AM EST
January 7, 2014

This webinar will provide an overview of the use of impedance (both simple and complex) to detect cell morphological changes. Emphasis will be placed on the use of different AC frequencies to distinguish cell parameters. To view a pre-recorded video of this webinar click here: ECIS Theory(22:55)

Cell Invasion / Extravasation Assays – 11:00 AM EST
January 21, 2014

This webinar will review ECIS as it is used to monitor endothelial monolayers as they are challenged with metastatic cell lines. Extravasation of these layers can be followed in real time to provide quantitative data regarding the metastatic potential of the cancer cells. To view a pre-recorded video of this webinar click here: Cell Invasion Assays (9:19)

Automated Cell Migration – 11:00 AM EST
February 4, 2014

The ECIS Wound Healing Assay replaces the traditional "scratch" or "scrape" assay. Instead of disrupting the cell layer mechanically with a needle or pipette tip and following the migration of cells to "heal" the wound with a microscope, we employ electric signals to both wound and monitor the healing process. We will also describe a novel and new approach to this measurement called the “electric fence”.  This webinar will review the basic principles of ECIS and then present quantitative data regarding these automated assays. To view a pre-recorded video of this webinar click here: Cell Migration (10:27)
 
Barrier Function Assays – 11:00 AM EST
February 18, 2014

This webinar will review ECIS and then show how complex impedance measurements can be used to evaluate the barrier function (permeability) of confluent endothelial monolayers. The ability of ECIS data to be mathematically modeled will be briefly introduced.

Real-time Electroporation and Monitoring – 11:00 AM EST
March 11, 2014

ECIS can be used to electroporate cells and then to immediately follow the results of the electroporation in real-time. This unique feature of the ECIS technology will be described in this webinar and sample data presented. To view a pre-recorded video of this webinar click here: Electroporation (10:28)

Cell Attachment and Spreading Measurements – 11:00 AM EST
March 25, 2014

The webinar will review ECIS and then present quantitative data gathered in real time regarding the attachment and spreading behavior of cells upon extracellular matrix proteins. 

Signal Transduction Assays – 11:00 AM EST
April 8, 2014

Data will be presented showing the ability of ECIS to monitor signal transduction in label-free, whole-cell assays. The morphological changes responsible for the impedance changes will be further evaluated by modeling the complex impedance data. To view a pre-recorded video of this webinar click here: Signal Transduction Assays (7:16)

Toxicology with ECIS – 11:00 AM EST
April 22, 2014

This webinar will review ECIS and then present data showing how ECIS impedance measurements respond to varying concentrations of toxic compounds.

ECIS Theory – 11:00 AM EST
May 6, 2014

This webinar will provide an overview of the use of impedance (both simple and complex) to detect cell morphological changes. Emphasis will be placed on the use of different AC frequencies to distinguish cell parameters. To view a pre-recorded video of this webinar click here: ECIS Theory(22:55)

Cell Invasion / Extravasation Assays – 11:00 AM EST
May 20, 2014

This webinar will review ECIS as it is used to monitor endothelial monolayers as they are challenged with metastatic cell lines. Extravasation of these layers can be followed in real time to provide quantitative data regarding the metastatic potential of the cancer cells. To view a pre-recorded video of this webinar click here: Cell Invasion Assays (9:19)

Automated Cell Migration – 11:00 AM EST
June 10, 2014

The ECIS Wound Healing Assay replaces the traditional "scratch" or "scrape" assay. Instead of disrupting the cell layer mechanically with a needle or pipette tip and following the migration of cells to "heal" the wound with a microscope, we employ electric signals to both wound and monitor the healing process. We will also describe a novel and new approach to this measurement called the “electric fence”.  This webinar will review the basic principles of ECIS and then present quantitative data regarding these automated assays. To view a pre-recorded video of this webinar click here: Cell Migration (10:27)

Barrier Function Assays – 11:00 AM EST
June 24, 2014

This webinar will review ECIS and then show how complex impedance measurements can be used to evaluate the barrier function (permeability) of confluent endothelial monolayers. The ability of ECIS data to be mathematically modeled will be briefly introduced.

Real-time Electroporation and Monitoring – 11:00 AM EST
July 8, 2014

ECIS can be used to electroporate cells and then to immediately follow the results of the electroporation in real-time. This unique feature of the ECIS technology will be described in this webinar and sample data presented. To view a pre-recorded video of this webinar click here: Electroporation (10:28)

Cell Attachment and Spreading Measurements – 11:00 AM EST
July 22, 2014

The webinar will review ECIS and then present quantitative data gathered in real time regarding the attachment and spreading behavior of cells upon extracellular matrix proteins. 

Signal Transduction Assays – 11:00 AM EST
September 9, 2014

Data will be presented showing the ability of ECIS to monitor signal transduction in label-free, whole-cell assays. The morphological changes responsible for the impedance changes will be further evaluated by modeling the complex impedance data. To view a pre-recorded video of this webinar click here: Signal Transduction Assays (7:16)

Toxicology with ECIS – 11:00 AM & 2:00PM EST
September 23, 2014

This webinar will review ECIS and then present data showing how ECIS impedance measurements respond to varying concentrations of toxic compounds.

ECIS Theory – 11:00 AM EST
October 7, 2014

This webinar will provide an overview of the use of impedance (both simple and complex) to detect cell morphological changes. Emphasis will be placed on the use of different AC frequencies to distinguish cell parameters. To view a pre-recorded video of this webinar click here: ECIS Theory(22:55)

Cell Invasion / Extravasation Assays – 11:00 AM EST
October 21, 2014

This webinar will review ECIS as it is used to monitor endothelial monolayers as they are challenged with metastatic cell lines. Extravasation of these layers can be followed in real time to provide quantitative data regarding the metastatic potential of the cancer cells. To view a pre-recorded video of this webinar click here: Cell Invasion Assays (9:19)

Automated Cell Migration – 11:00 AM EST
November 4, 2014

The ECIS Wound Healing Assay replaces the traditional "scratch" or "scrape" assay. Instead of disrupting the cell layer mechanically with a needle or pipette tip and following the migration of cells to "heal" the wound with a microscope, we employ electric signals to both wound and monitor the healing process. We will also describe a novel and new approach to this measurement called the “electric fence”.  This webinar will review the basic principles of ECIS and then present quantitative data regarding these automated assays. To view a pre-recorded video of this webinar click here: Cell Migration (10:27)

Barrier Function Assays – 11:00 AM EST
November 18, 2014

This webinar will review ECIS and then show how complex impedance measurements can be used to evaluate the barrier function (permeability) of confluent endothelial monolayers. The ability of ECIS data to be mathematically modeled will be briefly introduced.

Real-time Electroporation and Monitoring – 11:00 AM EST
December 9, 2014

ECIS can be used to electroporate cells and then to immediately follow the results of the electroporation in real-time. This unique feature of the ECIS technology will be described in this webinar and sample data presented. To view a pre-recorded video of this webinar click here: Electroporation (10:28)