ECIS Cultureware™ Disposable Electrode Arrays

ECIS Cultureware consists of sterile disposable electrode arrays containing gold film electrodes delineated with an insulating film. The well top assembly is made of polystyrene. The gold layer is thin enough to allow microscopic observation of the cells using a standard inverted tissue culture microscope. The ECIS electrode array is placed in an array holder located in the incubator.

 

 

96 Well Arrays

Electrodes per Well

Electrode Area (mm²)

Maximum Number of Cells Measured

Well Volume (µL)

96W1E+ PET

2

0.256

100-200

300

96W10idf PET

idf

2.09

2000-4000

300

96W20idf PET

idf

3.985

4000-8000

300

 

Standard 8 Well Arrays

Electrodes per Well

Electrode Area (mm²)

Maximum Number of Cells Measured

Well Volume (µL)

8W1E PET or PC

1

0.049

50-100

600

8W10E PET or PC

10

0.49

500-1000

600

8W10E+ PET or PC

40

1.96

2000-4000

600

m8W10idf PET

idf

2.09

2000-4000

300

8W20idf PET

idf

3.985

4000-8000

600

PET: Polyethylene terephthalate, standard thickness 0.25mm
PC: Clear polycarbonate substrate, standard thickness 0.51mm
(high numerical aperture (HNA) series with a thickness of 0.13mm is available- contact factory to order)

 

Specialty Arrays

Electrodes per Well

Electrode Area (mm²)

Maximum Number of Cells Measured

Well Volume (µL)

8W2x1E PET or PC

2x1

2 x 0.049

50-100

600

8W1CXE PET or PC

1

0.049

50-100

600

8W2LE PET or PC

2

0.20

200-400

600

8Wµ1E+ PET or PC

4

0.196

200-400

600

2W4x10E PC

4x10

4 x 0.49

2000-4000

600

*Specialty arrays may require 2-4 weeks lead time

 

Flow Arrays

Electrodes per Well

Electrode Area (mm²)

Number of Cells Measured when Confluent

Channel/Reservoir Volume (µL)

Channel Height
x width (mm)

1F8x1E PC

8x1 (1 channel)

0.049

50-100

90/60

0.36 x 5

1F8x10E PC

8x10 (1 channel)

0.49

500-1000

90/60

0.36 x 5

6F1E PC

1 (6 channels)

0.049

50-100

45/60

0.66 x 5

6F10E PC

10 (6 channels)

0.49

500-1000

45/60

0.66 x 5

1F2Y8x10E PC

8x4x2
(30&45 degree sides**)

0.49

500-1000

165/60

0.66 x 5

**Four electrodes are equal in size to 8W10E

 

“When ordering, please specify Array Name and Base Type"

PET: Polyethylene terephthalate, standard thickness 0.25mm
PC: Clear polycarbonate substrate, standard thickness 0.51mm
(high numerical aperture (HNA) series with a thickness of 0.13mm is available- contact factory to order)

 

Bar Code

All arrays include a bar code (code 128) label and serial number. The ECIS software embeds
the type of array and serial number information in the ECIS data file. The information can be scanned
in via a bar code scanner or keyed in manually. Requires ECIS software V 1.2.135 or later.

 

Application Notes

Concentration gradients inside a flow u-slide (ibidi)

Shear stress and shear rates for all channel flow u-slides (ibidi)

Avoiding Evaporation (ibidi)

Shear stress and shear rates for flow u-slides y-shaped (ibidi)

ECIS 8W TransFilter Application Note

ECIS Application Note - Cell-ECM Interactions

ECIS School Agenda

ECIS Operation Manual

ECIS Handbook

Phase-Contrast & Fluorescence Microscopy Tutorial

Adding Compounds to Study their Effect on ECIS Measurements

An Alternative Way to Obtain Good Cell Distribution on ECIS Arrays

Append and Combine Datasets

Choice of Program for ECIS Time Course Data Acquisition

Don't Forget Capacitance

Don't Overlook Micromotion

Improving Experiment Repeatability with Cysteine

Inoculation of Arrays

Pre-Coating Electrodes with Defined Protein Coats

 

 

96 Well Arrays (Download List of 96 Well Arrays)

 

96W1E+ PET

Each of the 96 wells in a standard plate configuration contains two circular 350 μm diameter active electrodes on a transparent PET substrate (measuring from 100-200 cells). As with other 1E arrays, a major use of this array is for the ECIS wound-healing assays where the  small electrodes assures the high current pulse will result in complete cell killing.

Only a small population of cells is monitored on the small electrodes resulting in a fluctuating impedance signal due to the random like movement of the cells (micromotion).

Applications include:

Cell Migration

Measurement of micromotion

Cell-ECM protein interactions

Signal transduction assays

Detection of invasion of endothelial cell layers by metastatic cells

Barrier function

In situ Cell Electroporation and Monitoring

 

 

96W10idf PET

Each of the 96 wells has an Inter-digitated finger configuration. The total electrode area is 2.09mm2 which measures a maximum of 2000-4000 cells.

Applications include:

Cell-ECM protein interactions

Signal transduction assays

Detection of invasion of endothelial cell layers by metastatic cells

Barrier function

Cell proliferation

 

 

96W20idf PET

Each of the 96 wells has an Inter-digitated finger configuration. The total electrode area is 3.985mm2 which measures a maximum of 4000-8000 cells.

Applications include:

Cell-ECM protein interactions

Signal transduction assays

Detection of invasion of endothelial cell layers by metastatic cells

Barrier function

Cell proliferation

 

 

Standard 8 Well Arrays (Download List of 8 Well Arrays)

 

8W1E PET or PC

Each of the 8 wells contains a single circular 250μm diameter active electrode. Each well has a substrate area of 0.8 cm2 and a maximum volume of 600μL. On average, with a confluent cell layer, approximately 50 to 100 cells will be measured by the electrode, but even a single cell can be observed.

Applications include:

Barrier function

Signal transduction assays

Cell Invasion

In situ Cell Electroporation and Monitoring

Cell migration / Wound Healing

Correlated microscopy and ECIS experiments

 

 

8W10E PET or PC

Each of the 8 wells contains ten circular 250 μm diameter active electrodes connected in parallel on a common gold pad. Each well has a substrate area of 0.8 cm2 and a maximum volume of 600 μL. On average, with a confluent cell layer, approximately 500 to 1000 cells will be measured by the electrodes.

Applications include:

Cell Attachment and Spreading

Cell proliferation

Cell Differentiation

Barrier function

Signal transduction assays

Cell Invasion

Cytotoxicity

 

 

8W10E+ PET or PC

Each of the 8 wells has two sets of 20 circular 250 μm diameter active electrodes located on inter-digitated fingers to provide measurements of cells upon a total of 40 electrodes. Each well has a substrate area of 0.8 cm2 and a maximum volume of 600 μL. On average, with a confluent layer, approximately 2000 to 4000 cells will be measured by the electrodes.

The 10E+ arrays are designed to monitor larger numbers of cells, sampling over the entire bottom of the well. Because of the relatively high number of cells, impedance fluctuations due to micromotion are smoothed out and do not obscure subtle changes in impedance due to the experimental conditions.

Applications include:

Cell Attachment and Spreading

Cell proliferation

Cell Differentiation

Cell-ECM Protein Interactions

Barrier function

Signal transduction assays

Cell Invasion

Cytotoxicity

 

 

m8W10idf PET

Our "mini" version of the 96W10idf array has 6 mm diameter wells for a substrate area of only 0.28cm2 and a maximum well capacity of 200 microliters. The smaller well size means that less reagents and cells are required for experimental runs. The total electrode area is 2.09mm2 which measures a maximum of 2000-4000 cells

Like other 10idf arrays, the mini array is designed to monitor a large number of cells, reducing impedance fluctuations due to random cell movements (micromotion), and samples cells over the entire well bottom. This affordable array is a good option for researchers using expensive cells or reagents. The m8W10idf mini-array is supported by software version 1.2.70 or higher and is sold in quantities of 12.

Applications include:

• Cell-ECM protein interactions

Signal transduction assays

• Detection of invasion of endothelial cell layers by metastatic cells

Barrier function

Cell proliferation

• Cytotoxicity

 

 

8W20idf PET

Each of the 8 wells has a total electrode area of 3.985mm2 located on inter-digitated fingers to provide measurements of cells. Each well has a substrate area of 0.8cm2 and a maximum volume of 600 μL. On average, with a confluent layer, approximately 4000 to 8000 cells will be measured by the electrodes.

The 8W20idf PET arrays are designed to monitor larger numbers of cells, sampling over the entire bottom of the well. Because of the relatively high number of cells, impedance fluctuations due to micromotion are smoothed out and do not obscure subtle changes in impedance due to the experimental conditions.

Applications include:

Cell Attachment and Spreading

Cell proliferation

Cytotoxicity

 

 

Specialty Arrays

 

8W2x1E PET or PC

This array is also called the Medusa array. Each well in this array has two independent single 250 µm diameter active electrodes. The Medusa array is useful for duplicating readings in the same well or to wound/electroporate one electrode while leaving the other as a control within the same well.

When connected to the array holder only the upper four wells are measured. To use the other four wells, the array is turned around and the contact pads at the other end are connected.

Applications include:

Barrier function

Signal transduction assays

Cell Invasion

In situ Cell Electroporation and Monitoring

Cell migration / Wound Healing

Correlated microscopy and ECIS experiments

 

 

8W1CXE PET or PC

This array is used to monitor the movement of cells in response to chemical gradients and is the array used in chemotaxis measurements first described by Hadjout, N. et al. (2001) Biotechniques 31 (5) 1130. The measuring electrode in this array is a thin gold line between two registry marks.*

Each well has a substrate area of 0.8 cm2 and a maximum volume of 600 μL. On average, with a confluent layer, approximately 50 to 100 cells will be monitored by the electrode.

*The gold line has the same total area as a 250 μm single circular electrode.

Applications include:

Cell Chemotaxis

 

 

8W2LE PET or PC

Each of the 8 wells contains a single linear electrode with dimensions of 667µm x 150µm and a measurement value equal to that of our standard 250µm circular electrodes. Each well has a substrate area of 0.8cm2 and a maximum volume of 600μL. On average, with a confluent cell layer, approximately 200 to 400 cells will be measured by the electrode, but even a single cell can be observed.

Applications include:

Cell migration / Wound Healing

Correlated microscopy and ECIS experiments

 

 

8Wµ1E+ PET or PC

Each of the 8 wells contains four 250µm circular electrodes which will measure from 200-400 cells. The placement of the electrodes at the center of the well allows for the use of cloning cylinders to be placed around the electrodes creating microwells. The area outside of the cloning cylinder can then be flooded to reduce evaporation from within the micro-wells.

Applications include:

Barrier function

Signal transduction assays

Cell Invasion

In situ Cell Electroporation and Monitoring

Cell migration / Wound Healing

Correlated microscopy and ECIS experiments

 

 

2W4x10E PC

Each of the 2 circular 25mm diameter wells contain four independent sets of ten 250 μm diameter active electrodes measuring from 2000-4000 cells. In addition, the 2W4x10E array is useful for duplicating readings in the same well or to wound/electroporate one electrode while leaving the other as a control within the same well.

Applications include:

Cell Attachment and Spreading

Cell proliferation

Cell Differentiation

Barrier function

Signal transduction assays

Cell Invasion

Cytotoxicity

Correlated microscopy and ECIS experiments

 

 

Flow Arrays

 

 

 

1F8x1E PC

This is a specialized Flow array having 8 active 250 μm diameter electrodes (each measuring from 50-100 cells) located in the central region at the base of a flow channel measuring 50mm in length 5mm in width and available in 0.36mm in height with a total channel volume of 90 μL.

Our flow arrays are designed for ECIS measurements of cells under perfused conditions or to mimic the shear stress endothelial cells experience in vivo or under flow mimicking the shear stress endothelial cells experience in vivo.

Applications include:

Barrier function

Signal transduction assays

Cell Invasion

In situ Cell Electroporation and Monitoring

Cell migration / Wound Healing

Cell proliferation

Cell Differentiation

Cytotoxicity

 

 

1F8x10E PC

This is a specialized Flow array having 8 sets of 10 active 250 μm diameter electrodes (each measuring from 500-1000 cells) located in the central region at the base of a flow channel measuring 50mm in length 5mm in width and available in 0.36 mm in height with a total channel volume of 90 μL.

Our flow arrays are designed for ECIS measurements of cells under perfused conditions or to mimic the shear stress endothelial cells experience in vivo or under flow mimicking the shear stress endothelial cells experience in vivo.

Applications include:

Barrier function

Signal transduction assays

Cell Invasion

In situ Cell Electroporation and Monitoring

Cell migration / Wound Healing

Cell proliferation

Cell Differentiation

Cytotoxicity

 

 

6F1E PC

This flow array allows 6 independent flow assays to be run simultaneously. The channels are 0.66mm in height and 5mm wide with 1 active 250um diameter electrode (measuring from 50-100 cells) per channel.

Each channel has a 45μL volume with 60μL reservoirs.

Applications include:

Barrier function

Signal transduction assays

Cell Invasion

In situ Cell Electroporation and Monitoring

Cell migration / Wound Healing

Cell proliferation

Cell Differentiation

Cytotoxicity

 

 

6F10E PC

This flow array allows 6 independent flow assays to be run simultaneously. The channels are 0.66mm in height and 5mm wide with 10 active 250um diameter electrodes (each measuring from 500-1000 cells) per channel.

Each channel has a 45μL volume with 60μL reservoirs.

Applications include:

Barrier function

Signal transduction assays

Cell Invasion

In situ Cell Electroporation and Monitoring

Cell migration / Wound Healing

Cell proliferation

Cell Differentiation

Cytotoxicity

 

 

1F2Y8x10E PC

This flow array is intended for bifurcation studies and blood vessel simulation. It splits into 30 degree Y channels in one direction and 45 degree Y channel in the other direction.

This array is double ended with 8 measurement channels available at each end. Eight measurement points, each with 4 circular active electrodes (with an area of 0.49mm2 measuring from 500-1000 cells, the area is the same as a 10E electrode), are located along the channel and through the Y portion of the channel. One end of the array is used to monitor the 30 degree Y channel and the other end is used to monitor the 45 degree Y channel. The electrodes are located close in the corners of the flow direction transition points. Each channel has a 165μL volume with 60μL reservoirs. The flow is always laminar, i.e., turbulent flows are not possible. For simulation of turbulence flow we recommend oscillating the flow. Defined shear stress and shear rate levels.

Recommended for the following applications under shear stress conditions:

Simulation of the bifurcation of blood vessels for arteriosclerosis research

Rolling and adhesion of leukocytes on endothelial cells cultured under flow

Cell-cell interaction studies and cell-drug interaction screenings under flow conditions

 

 

 

 

Custom Arrays

Custom arrays are alvailable. If you are interested in other electrode sizes and configurations, please contact us. We will be happy to work with you on our specific needs.

 

 

How to Order Arrays

We maintain a large supply of arrays for shipment from our facility. When arrays are ordered, a final oxygen plasma etching procedure is used to both clean the gold surfaces and to sterilize the arrays. They are then packed and mailed on the same day via FedEx 2nd day. Credit cards are accepted.

It is best to order electrode arrays in quantities such that they are not stored for periods of many months. We recommend storage for up to two months.

For array ordering and questions please contact Catherine Toniatti at (518) 880-6860. You may also send an
email to ctoniatti@biophysics.com or fax your order with contact numbers and all shipping & billing addresses to 518-880-6865.

All arrays can be ordered without upper chambers (NC). Gold films are transparent and the upper chambers can be removed following ECIS measurements to facilitate staining including fluorescence measurements.