ECIS trans-Filter Adapter
(ECIS Trans-Filter Adapter Application Note)

The ECIS® trans-Filter Adapter is designed to measure the TEER of cell layers grown on 24 well membrane filter inserts available from a broad range of manufacturers. The trans-Filter Adapter accepts up to eight 6.5mm dia. cell culture inserts. The trans-Filter Adapter is comprised of three parts, the base with contact pads and individually addressed electrodes, the common gold-plated stainless steel common dipping electrodes , and a clear lid.

Cell culture inserts fit into the wells of the base and the dipping electrode assembly are inserted into the cell culture inserts and rest on three gold pins that stick out of the base. The clear lid covers the wells to keep the contents sterile during transport from the tissue culture hood to the incubator.

The trans-Filter Adapter fits into one side of the ECIS® 16 Well Array Stations and two trans-Filter Adapters may be run simultaneously. A trans-Adapter Array and a normal 8W array may also be run together. Data acquisition proceeds as normal using the ECIS® Software.