How can TEER assays for barrier function be made easier and more accurate? By making them automated and reporting measurements in real time. The all new TEER96 by Applied Biophysics, Inc. A more effective approach to a traditionally complicated assay.
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The ECIS TEER96 is designed to carry out traditional trans-epithelial/endothelial electrical resistance measurements using ECIS (electric cell-substrate impedance sensing) technology to monitor the barrier function of cell layers grown on membrane insert filters. Data is collected continuously from up to 96 independent wells and reported as real-time barrier function changes in ohm-cm2.
Figure 1: Continuous TEER measurements taken on MDCK II cells that were already confluent (turquoise) and freshly inoculated (magenta) at time 0 in the ECIS® TEER96. The cells were inoculated in a 96-well cell-culture insert plate.
Non-invasive measurements may be made continuously for days with TEER96. The fixed position of electrodes throughout the measurements eliminates operator variability allowing precise and repeatable measurements of cell layers.
The TEER96 accepts standard Millipore® and Corning® 96-well filter plates. These are placed on the platen of the TEER96. An optical switch senses the array is in place and automatically moves the array into the station and precisely moves the plate up to the dipping pins. Each well has two stainless steel coated dipping pins, one for the counter electrode and one over the filter to take the measurement.
The system can take just one reading of each well per plate or take continuous data on a plate over days or weeks. The system can be programmed to include a wash cycle after a certain number of arrays are run. The system includes a bar code reader, the bar code data is integrated with the data file.