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TEER24


Barrier Function Measurement System

Description


How can TEER assays for barrier function be made easier and more accurate? By making them automated and reporting measurements in real time. The all new TEER24 by Applied Biophysics, Inc. A more effective approach to a traditionally complicated assay.

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Overview


The ECIS TEER24 is designed to carry out traditional trans-epithelial/endothelial electrical resistance measurements using ECIS (electric cell-substrate impedance sensing) technology to monitor the barrier function of cell layers grown on membrane insert filters. Data is collected continuously from up to 24 independent wells and reported as real-time barrier function changes in ohm-cm2.

Benefits
  • - Automated TEER measurements from under 10 to 1,000 ohm-cm2 in 24 wells
  • - Uses standard commercially available membrane inserts
  • - Data recorded and visualized in real time under incubator conditions
  • - Accurate measuring of endothelial barrier function
  • - Long term data collection capabilities
  • - Easily sterilizable, disposable array and autoclavable stainless dipping pins
  • - Fast barrier function dynamics monitoring
  • - Non-invasive & label free
  • - Highly reproducible
  • - User friendly software
  • - Data output in CSV or graphical (jpg, tif)
  • - Create figures and plots of data
System Includes:
  • - 24 well channel station located in CO2 incubator
  • - External control module
  • - Laptop PC
  • - ECIS control, acquisition, and display software
  • - Validation test array
  • - 1 Common Electrode Array (CEA) with 24 gold pins
  • - Six 24-well TEER consumable electrode microplates

Non-invasive measurements may be made continuously for days and even weeks with TEER24. The fixed position of electrodes throughout the measurements eliminates operator variability allowing precise and repeatable measurements of cell layers with weak barrier function (<10 ohm-cm2). There is no need to take the station out of the incubator, therefore eliminating temperature variations.

The TEER24 accepts standard 24 well membrane inserts from any commercial supplier. These are placed in a disposable sterile baseplate with addressable electrodes on the well bottom. Once medium and cell suspension are added, an autoclavable array of gold dipping electrodes is inserted and measurements may begin. Even with the dipping electrodes in place, the media both in and out of the inner well can be easily reached with standard micropipettes.

  • - 24 well microplates use standard 6mm filters
  • - 24 well plates are barcoded with serial numbers for tracking
  • - 24 well dipping assembly is medical grade stainless and autoclavable
  • - Gold electrodes
  • - 75 Hz sinusoidal signal
  • - Power: 3 watts; 12 V dc
  • - Station: 25.5 x 18 x 4.5 cm, 2.3 kg
  • - Controller: 21.5 x 18 x 4.5 cm, 2 kg
  • - Connects to laptop via USB
  • - Windows 10 OS

Data Sheets