The ECIS TEER24 is designed to carry out traditional trans-epithelial/endothelial electrical resistance measurements using ECIS (electric cell-substrate impedance sensing) technology to monitor the barrier function of cell layers grown on membrane insert filters. Data is collected continuously from up to 24 independent wells and reported as real-time barrier function changes in ohm-cm2.
Non-invasive measurements may be made continuously for days and even weeks with TEER24. The fixed position of electrodes throughout the measurements eliminates operator variability allowing precise and repeatable measurements of cell layers with weak barrier function (<10 ohm-cm2). There is no need to take the station out of the incubator, therefore eliminating temperature variations.
The TEER24 accepts standard 24 well membrane inserts from any commercial supplier. These are placed in a disposable sterile baseplate with addressable electrodes on the well bottom. Once medium and cell suspension are added, an autoclavable array of gold dipping electrodes is inserted and measurements may begin. Even with the dipping electrodes in place, the media both in and out of the inner well can be easily reached with standard micropipettes.