In terms of in vitro experiments, it is paramount in the scientific community to mimic the natural environment to the best of our abilities. In the case of endothelial cells, providing fluid dynamics to the experiments brings us closer to allowing the cells to behave as they would in the vascular system. That’s why Applied Biophysics is now providing the ECIS p-Flow Peristaltic Pump addition to the ECIS system to collect automated, real-time data of endothelial cells under fluidic shear stress, bringing new meaning to “go with the flow”.Download Data Sheet
The ECIS Z Theta instrument can be equipped with the ECIS p-Flow Peristaltic Pump option specifically designed for researchers who wish to employ ECIS measurements to study the behavior of cells under flow conditions. A variety of flow arrays are available: a single channel (0.4 x 5 x 50mm) which has 8 electrodes, a six channel with one electrode per channel, and a "Y" channel to simulate vessel branching. Various combinations of channel heights and types of electrodes (1E or 10E) are also available.
Flow can be programmed from slow to high rates yielding shear stress similar to that experienced by endothelial cells in differing areas of the vascular system in vivo. In addition to steady laminar flow, the flow programming also includes the ability to generate pulsed flows of different periods and with varying flow rates.
Although only one or two pumps are required for many ECIS applications, multiple pumps can be connected together via a USB hub, all controlled independently by the software. The ECIS Z Theta instruments can also be equipped with an elevated field module which allows for electroporation and/ or cell migration measurements, via automated wound-healing assays, under flow conditions.
The Model ECIS p-Flow can be controlled manually to adjust the rate of flow rate from 70 ul/min to 20 ml/min. Direction of the pump can also be adjusted via a toggle switch or controlled automatically via the ECIS software to change the direction of the flow.