Products

ECIS Zθ (Theta) Data Output

Z v. T: Impedance v. Time

ECIS Z provides researchers with an advanced, automated, non-invasive means to monitor cell behavior in real-time and without the use of labels.

Used for general morphological changes in cells. Response of cells to viral infection is shown.1



R v. T: Resistance v. Time

ECIS Z Used for general morphological changes in cells. Also used to quickly measure cell-cell interaction. Left, data showing dose response of BPAEC cells to Sph-1-P or thrombin.

Used for general morphological changes in cells. Also used to quickly measure cell-cell interaction. Left, data showing dose response of BPAEC cells to Sph-1-P or thrombin.2



Rb Data

ECIS Z Rb Data

Mathematical modeling can be called up to monitoring changes in barrier function as shown above following the addition of VEGF to endothelial cells.3



C v. T: Capacitance v. Time

ECIS Z Monitoring the dynamics of cell attachment & spreading. Capacitance plots are also used to determine and measure cell confluence and proliferation.

Monitoring the dynamics of cell attachment & spreading. Capacitance plots are also used to determine and measure cell confluence and proliferation.4



3D Data: Resistance v. Frequency v. Time

ECIS Z Monitoring the dynamics of cell attachment & spreading. Capacitance plots are also used to determine and measure cell confluence and proliferation.

3D plots allow researchers to visualize time course data at multiple frequencies providing a third axis of analysis for informative trend observation.5





1: Z v. T: Courtesy of Dr. Ivar Giaever, Applied BioPhysics.
2: R v. T: Garcia, J.G.N., Liu, F., Verin, A.D., Birukova, A., Dechert, M.A., Gerthoffer, W.T., Bamberg, J.R., English, D., “Sphingosine 1-phosphate promotes endothelial cell barrier integrity by Edg-dependent cytoskeletal rearrangement” J Clin Invest. 108(5): 689-701, (2001).
3: C v. T: Wegener, Joachim. Keese, R., Charles, Giaever, Ivar, “Electric Cell-Substrate Impedance Sensing (ECIS) as a Noninvasive Means to Monitor the Kenetics of Cell Spreading to Artificial Surfaces” Experimental Cell Research 259, 158-166 (2000).
4: Rb Data: Becker,Patrice M., Verin, Alexander D., Booth, Mary Ann, Liu,Feng, Birukova, Anna and Garcia, Joe G. N., “Differential regulation of diverse physiological responses to VEGF in pulmonary endothelial cells”, Amer J. Physiol., 281: L1500-L1511, (2001).
5: 3D Plot: Courtesy of Dr. Charles Keese, Applied BioPhysics.