News & Events
News & Events
Press Release
HTS- Automated, Real-time, Cell Monitoring with ECIS
July 1, 2007…… Applied BioPhysics, an analytical instrument manufacturer in Troy, NY, introduces a new High Throughput automated instrument to monitor cell behavior quantitatively, in real time.
The ECIS 9600™ is a high throughput instrument, designed to monitor cells grown in special arrays having a standard 96 well plate configuration and footprint. The consumable 96 well arrays (96W1E) are affordable and easily connected to the instrumentation using a single lever to make electrical contact. The instrument is capable of scanning all 96 wells in approximately 30 seconds making impedance measurements at a fixed AC frequency (three frequencies are available).
Like other ECIS instruments, using very weak AC current, the impedance of a small active electrode in each well is measured in real time to provide nearly immediate indications of cell activity and response to agents.
In addition to noninvasive measurements, the ECIS 9600™ can also be programmed to applied elevated fields for cell migration measurements via an automated wound-healing assay [PNAS 101: 1554-1559 (2004)]. The same high fields can also be used at shorter duration to electroporate membrane-impermeable compounds into cells for analysis. All data acquisition and analysis is handled with user-friendly software running on a Windows XP Professional platform. Data can also be readily converted to text files for export to other data analysis systems.
In addition to cell migration assays, one can devise assays for the many ECIS applications such as: signal transduction, cell invasion, cell integrin-ECM interactions and in vitro toxicology.