IBCA 2013 in Budapest
IBCA 2011 in Regensburg, Germany
Professor Joachim Wegener hosted over 100 attendees from 16 different countries at the 1st Conference on Impedance-based Cellular Assays at the University of Regensburg in Regensburg, Germany.
Nobel Laureate Ivar Giaever addressed the group with a talk entitled, "The Future of Science?" followed by a variety of research presentations on topics including stem cell differentiation, endothelial cell barrier function, cancer cell assays and the toxicity of nano particles.
In addition to the platform talks, numerous researchers presented their latest data during a lively poster session prior to the conference dinner. An in-depth tutorial session kicked off the meeting with lectures that ranged from the physics of impedance-based measurements and the physical design of electrodes, to an overview of cellular applications.
Dr. Ivar Giaever is featured in local German newspaper:
2010 ECIS Users Meeting
The ECIS Users meeting, held August 24-27 at the historic Rensselaerville Institute in Upstate NY, was a great success both scientifically and socially. Over 40 attendees from the United States and Europe met for three days of talks, poster sessions and workshops. Research topics varied, but all had the common theme of employing ECIS measurements to discern changes in cell behavior. It is difficult to single out any particular talk, but one that stimulated much interest was the first description of the use of ECIS to follow adipose-derived stem cell differentiation.
On Wednesday evening, Ivar Giaever, CTO of Applied BioPhysics and recipient of the 1973 Nobel Prize in physics, gave a stimulating and humorous talk entitled "How to Win a Nobel Prize." You can watch the video of this talk by clicking on the link below:
"How to win a nobel prize" 31 minutes .FLV real time viewing
"How to win a nobel prize" 31 minutes .MP4 real time viewing at higher resolution on Windows 7 with Windows media player or Apple Quick Time Player (160 MB)
The Rensselaerville Institute staff provided delicious meals throughout the meeting including a wonderful Thursday night barbecue held outdoors under starry skies. In addition, each night there were fireside discussion sessions where ECIS users exchanged ideas, shared common experiences and formed new friendships.
We look forward to next year where tentative plans are in place for another ECIS Users Meeting in Regensburg, Germany in late July 2011. Stay tuned for details.
To view pictures of the meeting please visit our Facebook Page.
New ECIS Arrays
8WCP (Cell Proliferation) -- Each of the 8 wells has two sets of 7.5mm x 0.5mm rectangular active electrodes located on inter-digitated fingers to provide measurements of cells. Each well has a substrate area of 0.8 cm^2 and a maximum volume of 600 μL. On average, with a confluent layer, approximately 4000 to 8000 cells will be measured by the electrodes.
The 8WCP arrays are designed to monitor larger numbers of cells, sampling over the entire bottom of the well. Because of the relatively high number of cells, impedance fluctuations due to micromotion are smoothed out and do not obscure subtle changes in impedance due to the experimental conditions.
Recommended for use with Cell Proliferation Assays.
Line (Wounding) -- Each of the 8 wells contains a single linear electrode with dimensions of 667µm x 150µm and an area equal to that of our standard 250µm circular electrodes. Each well has a substrate area of 0.8 cm2 and a maximum volume of 600μL. On average, with a confluent cell layer, approximately 50 to 100 cells will be measured by the electrode, but even a single cell can be observed.
Recommended for use with cell migration measurements via automated wound healing.
2W4x10E -- This array configuration was developed in collaboration with Yang Wang of the University of Chicago. Dr. Wang's requirements were for arrays with a large surface area to volume ratio to allow for rapid gas exchange within the media. These arrays have greatly aided Dr. Wang in his ECIS anoxia experiments. Each of the 2 circular 25 mm diameter wells contain four independent sets of ten 250 μm diameter active electrodes. In addition, the center of these arrays are left clear, without gold or photo-resist allowing for live cell Fluorescent microscopy with a standard inverted microscope.
8F10E -- This is a specialized array with 8 sets of 10 active 250 μm diameter electrodes located in the central region at the base of a flow channel measuring 50 mm long, 5 mm in width and 0.4 mm in height.
The flow array is useful for ECIS measurements of cells under perfused conditions or to mimic the shear stress endothelial cells experience in vivo. The channel has an area of 2.5 cm2 and a channel volume of 100 μL. On average, a confluent layer of approximately 500 to 1000 cells will be monitored by each set of electrodes.
HNA Arrays -- We can customize any of our 8 well arrays with a thin substrate, the thickness of a coverslip. Recommended for use with High Numerical Aperture Microscopy.