ibidi u-Slide I
Combination of a cell culture dish and a cover slip for imaging inside a channel

ibidi u-Slide I
- 100 ul channel - small volume
- suited for immunofluorescence
- real time imaging during staining process
- available for elastic surface
- large observation area for microscopy
- high resolution microscopy of living and fixed cells
- lids for long term cultivation
Seeding Cells

Step 1:
fill the channel with 100 ul cell suspension
Step 2:
fill one reservoir with cell-free media; surface tension prevents flushing the cells from the channel
Step 3:
fill second reservois with the same amount of cell-free media to equilibrate the system.

catalog numbers:
ibiTreat, tissue culture treated, sterile
80106
coated (Collagen IV), sterile
80101
coated (Fibronectin), sterile*
80102
coated (Poly-L-Lysine), sterile
80110
coated (Poly-D-Lysne), sterile*
80115
hydrophobic, uncoated, sterile
80111
elastic surface ESS, 28 kPa, sterile*
80141
elastic surface ESS, coated (Collagen IV), sterile*
80142
*available on request only

technical details:
recommended vol/reservoir
600 ul
number of channels
1
volume of channel
100 ul
height of channel
0.4 mm
length of channel
50 mm
width of channel
5 mm
growth area
2.5cm2
cottom matches coverslip
No. 1.5