ibidi

u-Slide 2x9 well

Two chamber u-Slide, each chamber with 9 independent subdivisions for cell based assays

Two chamber u-slide on a microscope

u-Slide 2x9 well

  • co-culture experiments with different cell lines or primary cells
  • cells share soluble factors but grow seperated
  • open system for tissue engineering screenings
  • scratch assays with co-cultures
  • low evaporation
  • in situ cell experiments without cell transfer






Seeding Cells

ibidi u slide for co-culture experimentsStep 1:
Seed 60 ul of recipent cells in the inner reservoir

Step 2:
Seed 60 ul of the feeder cells in the outer reservoirs.

Await cell attachment; aspirate all unattached cells and media

Step 3:
Overlay the major well fully with 1.3 ml cell free media

















catalog numbers:
ibiTreat, tissue culture treated, sterile
81806
coated (Collagen IV), sterile
81802
coated (Fibronectin), sterile*
81803
coated (Poly-L-Lysine), sterile
81804
coated (Poly-D-Lysne), sterile*
81805
hydrophobic, uncoated, sterile
81801
*available on request only

two chamber ibidi slide for co-culture cell experimentstwo chamber ibidi slide for cell based assays

technical details:
number of wells
2
volume per major well
1.3 ml
dimensions of major wells (mm)
21.5x23.6x6.8
number of minor wells
2x9
volume of each minor well
70 ul
dimensions of minor wells
6.1x6.8x1.3
growth area per minor well
0.4 cm2
bottom matches coverslip
No. 1.5