Please have a look in the µ-Slide section on our website. There all the dimensions are given for all Slides.
The µ-Slides and µ-Dishes are optimized for inverted microscopes. The bottom matches No 1.5 coverslip thickness and is made from high quality plastic material. Autofluorescence, birefringence, and refractive index of the bottom are similar to those of glass allowing the use of all kinds of objective lenses (1x up to 100x oil immersion). Our slide optics are designed to be used with 0.17 mm corrected objective lenses.
(Source: Gerhard Menzel, Glasbearbeitungswerk GmbH & Co. KG, Braunschweig, Germany)
Our polymer has almost the same optical quality as glass.
The slides and dishes are made of biocompatible materials. The biocompatibility of all types of ibidi-slides and ibidi-dishes is tested regarding EN ISO 10993-5 (Test for cytotoxicity: in vitro methods)
comparable to standard cell culture flasks and Petri dishes. The adhesion of cells to ibiTreat µ-Slides is strong enough to perform flow experiments simulating the physiological shear stress of the blood flow. The surface was tested with over 20 different cell lines and primary cells.
We have been working with the following cell lines internally: HBE; HELA; RAT1, 3T3, NRK´s, HUVEC, CHO, COS7, 293, PC-12, B16, HCV29
Like most common plastics, the used plastic has a higher permeability for O2 and CO2 than glass. Also in the media, a lot of O2 is dissolved. Because of the height of the channel (µ-Slide I, µ Slide VI, µ-Slide y-shaped), enough O2 and CO2 is available for a long term experiments.
Applied BioPhysics, Inc. 185 Jordan Road Troy, New York 12180
Phone: 518-880-6860 1-866-301-ECIS (3247) Fax: 518-880-6865 Email: info@biophysics.com
ECIS: An automated instrument to monitor cell behavior
© 2009 Applied BioPhysics