The ECIS® 24W TransFilter Adapter is designed to measure the TEER of cell layers grown on 24 well membrane filter inserts available from a broad range of manufacturers. The 24W TransFilter Adapter accepts up to 24 6.5mm dia. cell culture inserts. The 24W TransFilter Adapter is comprised of three parts; the base with contact pads and individually addressed gold electrodes, the common gold-plated stainless steel dipping electrodes, and a lid.
Cell culture inserts fit into the wells of the base and the dipping electrode assembly are inserted into the cell culture inserts and rest on three gold pins that stick out of the base. The lid covers the wells to keep the contents sterile during transport from the tissue culture hood to the incubator. All the components can be autoclaved for sterilization.
The 24W TransFilter Adapter fits into the ECIS® 96 Well Array Station. A Z theta instrument with complex impedance capability is required to run the 24W TransFilter Adapter. Data acquisition proceeds as normal using the ECIS® Software.